ABSTRACT
Objective:To investigate the expressions of Mfn2(mitofusin 2) in non-small-cell lung cancer (NSCLC) tissues and non-cancerous lung tissues,and analyze its relationship with clinicopathological characteristics of lung carcinomas.Methods:The expressions of Mfn2 mRNA and protein in 92 cases of NSCLC tissues and 27 cases of non-cancerous lung tissues were detected by in situ hybridization and immunohistochemical methods. Results:The positive rates of Mfn2 mRNA and protein in pulmonary squamous cell carcinoma were higher than those in adenocarcinoma (83.33% and 89.58% vs 56.82% and 65.91%), respectively. The positive rates of Mfn2 mRNA and protein in NSCLC were higher than those in the non-cancerous lung tissues (25.93% and 29.63%) . The difference was statistically significant among the three groups (P0.05). The expression of Mfn2 mRNA was consistent with that of Mfn2 protein in NSCLC.Conclusion:Mfn2 was involved in the initiation and progression of lung cancer, and the expression of Mfn2 was related to the histological types of lung cancer and its differentiation degree.
ABSTRACT
Objective To understand the factors which would effect the quality of blood specimen,and then set the countermeasures. Methods Investigated the whole course of venous blood sampling by the quality control group,and then analyzed the data. Results 13% nursing personnel did not know the factors which would effect the quality of blood specimen.9% nursing personnel collect venous blood with a nonstandard way.The factors which would effect the quality of blood specimen were: test tube was not enough dry,the injector air leak,mechanical injury,the time of deliver specimen was too long,anticoagulant clotting,and so on. Conclusion Strengthen the training of professional skill and knowledge for nursing personnel,and then improve the quality of blood specimen.